Sample preparation for high-throughput sequencing

The famous PhD course on sample preparation for high-throughput sequencing is organized in Uppsala once again on 12-16 February 2018. 

Please sign up before 7/1 2018 via this link:  

Aim: This intensive course aims to provide students with practical training as well as theoretical background for collection, handling and preparation of samples for high-throughput DNA and RNA sequencing of environmental communities. Participants will become well acquainted with all practical steps and familiar with their theoretical background and main pitfalls. The course also offers students opportunities to discuss their own sampling and sequencing methods and bioinformatics tools. 

Content: The course consists of preparatory literature reading and a one-week meeting with short presentations of own work, lectures on theoretical background, and an intensive laboratory exercise (five half-days) where all steps in the sample preparation protocol will be trained, using soil fungal communities as an example. 

Preliminary program: 
Monday 12/2: Sampling and sample types, student presentations 
Tuesday 13/2: DNA extraction, inhibition 
Wednesday 14/2: PCR optimizations, primers, NGS technologies 
Thursday 15/2: preparation of multiplexed PCR amplicons 
Friday 16/2: Amplicon pooling and quality control 

Venue: Ultuna campus, Uppsala. 
Swedish University of Agricultural Sciences. 
Easy access from Arlanda Airport 

Funding available: The NEFOM (North European Forest Mycologists, funded by SNS/EFINORD) will fund travels and accommodation for a limited number of participants, giving priority to PhD students representing different NEFOM labs and based on motivation and application date. Accommodation will be in double rooms at Sunnersta Herrgård http://www.sunnerstaherrgard.se/bokning/ in walking distance from campus. 

More information here

For more information please contact: Karolina.jorgensen@slu.se  or Karina.clemmensen@slu.se